The Anti-Inflammatory and Antimicrobial Potential of Selected Ethnomedicinal Plants from Sri Lanka.
Mayuri NapagodaJana GerstmeierHannah ButschekSudhara De SoyzaSimona PaceSybille LorenzMallique QaderSanjeeva WitharanaAjith NagahawatteWeerasinghe Mudiyanselage Dilip Gaya Bandara WijayaratneAleš SvatošLalith JayasingheAndreas KoeberleOliver WerzPublished in: Molecules (Basel, Switzerland) (2020)
Traditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal plant species employed in Sri Lanka. The extracts were subjected to cell-based and cell-free assays of 5-lipoxygenase (5-LO), microsomal prostaglandin E2 synthase (mPGES)-1, and nitric oxide (NO) scavenging activity. Moreover, antibacterial and disinfectant activities were assessed. Characterization of secondary metabolites was achieved by gas chromatography coupled to mass spectrometric (GC-MS) analysis. n-Hexane- and dichloromethane-based extracts of Garcinia cambogia efficiently suppressed 5-LO activity in human neutrophils (IC50 = 0.92 and 1.39 µg/mL), and potently inhibited isolated human 5-LO (IC50 = 0.15 and 0.16 µg/mL) and mPGES-1 (IC50 = 0.29 and 0.49 µg/mL). Lipophilic extracts of Pothos scandens displayed potent inhibition of mPGES-1 only. A methanolic extract of Ophiorrhiza mungos caused significant NO scavenging activity. The lipophilic extracts of G. cambogia exhibited prominent antibacterial and disinfectant activities, and GC-MS analysis revealed the presence of fatty acids, sesquiterpenes and other types of secondary metabolites. Together, our results suggest the prospective utilization of G. cambogia as disinfective agent with potent anti-inflammatory properties.
Keyphrases
- anti inflammatory
- cell free
- endothelial cells
- nitric oxide
- gas chromatography
- staphylococcus aureus
- ms ms
- single cell
- induced pluripotent stem cells
- fatty acid
- mass spectrometry
- oxidative stress
- pluripotent stem cells
- high throughput
- tandem mass spectrometry
- cell therapy
- high resolution mass spectrometry
- climate change
- solid phase extraction
- bone marrow