Crystal structure of TEX101, a glycoprotein essential for male fertility, reveals the presence of tandemly arranged Ly6/uPAR domains.
Mamiko MasutaniShunya SakuraiToshiyuki ShimizuUmeharu OhtoPublished in: FEBS letters (2020)
Testis-expressed gene 101 (TEX101) is a glycosyl-phosphatidylinositol-anchored glycoprotein essential for sperm fertility and spermatogenesis. TEX101 interacts with lymphocyte antigen 6 complex, locus K (Ly6k) as well as a disintegrin and metallopeptidase domain 3 (ADAM3). Although these proteins are considered essential for fertility, the associated mechanisms remain uncharacterized. Herein, we determined the crystal structure of human and mouse TEX101, revealing that TEX101 contains two tandem Ly6/uPAR (LU) domains. Detailed structural analyses revealed characteristic surfaces of TEX101 that may be involved in the interactions with other proteins or membranes. These results provide the structural basis for the role of TEX101 in fertilization and could contribute to developing diagnostic methods and treatments for infertility or developing male contraceptives.
Keyphrases
- structural basis
- endothelial cells
- copy number
- genome wide
- pseudomonas aeruginosa
- insulin resistance
- staphylococcus aureus
- skeletal muscle
- young adults
- metabolic syndrome
- peripheral blood
- transcription factor
- protein kinase
- induced pluripotent stem cells
- candida albicans
- genome wide association study
- pluripotent stem cells