Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116.
Yajiao WuXiaoying ChenWenqiang BaoXinyu HongChutao LiJiatong LuDongcheng ZhangAn ZhuPublished in: Genes (2022)
Humantenine, an alkaloid isolated from the medicinal herb Gelsemium elegans (Gardner & Chapm.) Benth., has been reported to induce intestinal irritation, but the underlying toxicological mechanisms remain unclear. The object of the present study was to investigate the RNA N6-methyladenosine (m6A) modification and distinct mRNA transcriptome profiles in humantenine-treated HCT116 human colon cancer cells. High-throughput MeRIP-seq and mRNA-seq were performed, and bioinformatic analysis was performed to reveal the role of abnormal RNA m6A modification and mRNA expression in humantenine-induced intestinal cell toxicity. After humantenine treatment of HCT116 cells, 1401 genes were in the overlap of differentially m6A-modified mRNA and differentially expressed mRNA. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotation terms for actin cytoskeleton, tight junctions, and adherens junctions were enriched. A total of 11 kinds of RNA m6A methylation regulators were differentially expressed. The m6A methylation levels of target genes were disordered in the humantenine group. In conclusion, this study suggested that the HCT116 cell injury induced by humantenine was associated with the abnormal mRNA expression of m6A regulators, as well as disordered m6A methylation levels of target genes.
Keyphrases
- genome wide
- dna methylation
- single cell
- cell cycle arrest
- copy number
- rna seq
- binding protein
- high throughput
- endothelial cells
- genome wide identification
- cell death
- transcription factor
- cell therapy
- induced apoptosis
- poor prognosis
- blood brain barrier
- pluripotent stem cells
- working memory
- mesenchymal stem cells
- bioinformatics analysis
- pi k akt
- signaling pathway
- long non coding rna
- nucleic acid
- bone marrow
- newly diagnosed