Two Artificial Tears Outbreak-Associated Cases of Extensively Drug-Resistant Pseudomonas aeruginosa Detected Through Whole Genome Sequencing-Based Surveillance.
Alexander J SundermannVatsala Rangachar SrinivasaEmma G MillsMarissa P GriffithKady D WaggleAshley M AyresLora PlessGraham M SnyderLee H HarrisonDaria Van TynePublished in: The Journal of infectious diseases (2023)
We describe 2 cases of extensively drug-resistant Pseudomonas aeruginosa infection caused by a strain of public health concern, as it was recently associated with a nationwide outbreak of contaminated artificial tears. Both cases were detected through database review of genomes in the Enhanced Detection System for Hospital-Associated Transmission (EDS-HAT), a routine genome sequencing-based surveillance program. We generated a high-quality reference genome for the outbreak strain from an isolate from our center and examined the mobile elements encoding blaVIM-80 and bla-GES-9 carbapenemases. We used publicly available Pseudomonas aeruginosa genomes to explore the genetic relatedness and antimicrobial resistance genes of the outbreak strain.
Keyphrases
- drug resistant
- multidrug resistant
- pseudomonas aeruginosa
- acinetobacter baumannii
- public health
- klebsiella pneumoniae
- antimicrobial resistance
- genome wide
- cystic fibrosis
- biofilm formation
- healthcare
- adverse drug
- quality improvement
- gene expression
- single cell
- emergency department
- drinking water
- clinical practice
- staphylococcus aureus
- real time pcr
- copy number
- global health
- genome wide identification
- label free
- loop mediated isothermal amplification
- quantum dots