Sorption of Cationic Surfactants to Artificial Cell Membranes: Comparing Phospholipid Bilayers with Monolayer Coatings and Molecular Simulations.
Niels TimmerSteven T J DrogePublished in: Environmental science & technology (2017)
This study reports the distribution coefficient between phospholipid bilayer membranes and phosphate buffered saline (PBS) medium (DMW,PBS) for 19 cationic surfactants. The method used a sorbent dilution series with solid supported lipid membranes (SSLMs). The existing SSLM protocol, applying a 96 well plate setup, was adapted to use 1.5 mL glass autosampler vials instead, which facilitated sampling and circumvented several confounding loss processes for some of the cationic surfactants. About 1% of the phospholipids were found to be detached from the SSLM beads, resulting in nonlinear sorption isotherms for compounds with log DMW values above 4. Renewal of the medium resulted in linear sorption isotherms. DMW values determined at pH 5.4 demonstrated that cationic surfactant species account for the observed DMW,PBS. Log DMW,PBS values above 5.5 are only experimentally feasible with lower LC-MS/MS detection limits and/or concentrated extracts of the aqueous samples. Based on the number of carbon atoms, dialkylamines showed a considerably lower sorption affinity than linear alkylamine analogues. These SSLM results closely overlapped with measurements on a chromatographic tool based on immobilized artificial membranes (IAM-HPLC) and with quantum-chemistry based calculations with COSMOmic. The SSLM data suggest that IAM-HPLC underestimates the DMW of ionized primary and secondary alkylamines by 0.8 and 0.5 log units, respectively.
Keyphrases
- simultaneous determination
- ms ms
- molecular dynamics
- fatty acid
- solid phase extraction
- sewage sludge
- molecular dynamics simulations
- organic matter
- randomized controlled trial
- high performance liquid chromatography
- monte carlo
- mass spectrometry
- tandem mass spectrometry
- gas chromatography
- density functional theory
- emergency department
- liquid chromatography
- stem cells
- mesenchymal stem cells
- cell therapy
- single molecule
- magnetic resonance imaging
- electronic health record
- high resolution
- molecular docking
- label free
- deep learning
- diffusion weighted imaging
- artificial intelligence
- real time pcr