Genistein Improves Skin Flap Viability in Rats: A Preliminary In Vivo and In Vitro Investigation.
Lenka FáberIvan KováčPetra MitrengováMartin NovotnýLenka VarinskáTomáš VasilenkoMartin KelloMatúš ČomaTomáš KurucKlaudia PetrováIvana MiláčkováAnika KuczmannováVlasta PeržeľováŠtefánia MižákováErik DosedlaFrantišek SabolJán LuczyMilan NagyJaroslav MajerníkMartin KoščoPavel MučajiPeter GálPublished in: Molecules (Basel, Switzerland) (2018)
Selective estrogen receptor modulators (SERMs) have been developed to achieve beneficial effects of estrogens while minimizing their side effects. In this context, we decided to evaluate the protective effect of genistein, a natural SERM, on skin flap viability in rats and in a series of in vitro experiments on endothelial cells (migration, proliferation, antioxidant properties, and gene expression profiling following genistein treatment). Our results showed that administration of genistein increased skin flap viability, but importantly, the difference is only significant when treatment is started 3 days prior the flap surgery. Based on our in vitro experiments, it may be hypothesized that the underlying mechanism may rather by mediated by increasing SOD activity and Bcl-2 expression. The gene expression profiling further revealed 9 up-regulated genes (angiogenesis/inflammation promoting: CTGF, CXCL5, IL-6, ITGB3, MMP-14, and VEGF-A; angiogenesis inhibiting: COL18A1, TIMP-2, and TIMP-3). In conclusion, we observed a protective effect of genistein on skin flap viability which could be potentially applied in plastic surgery to women undergoing a reconstructive and/or plastic intervention. Nevertheless, further research is needed to explain the exact underlying mechanism and to find the optimal treatment protocol.
Keyphrases
- soft tissue
- endothelial cells
- genome wide
- wound healing
- randomized controlled trial
- estrogen receptor
- genome wide identification
- signaling pathway
- vascular endothelial growth factor
- breast reconstruction
- poor prognosis
- gene expression
- small molecule
- type diabetes
- coronary artery disease
- metabolic syndrome
- transcription factor
- adipose tissue
- skeletal muscle
- coronary artery bypass
- percutaneous coronary intervention
- cell migration
- smoking cessation