Ameliorative effect of Silybin on bisphenol A induced oxidative stress, cell proliferation and steroid hormones oxidation in HepG2 cell cultures.
Stefania LamaDaniela VanacoreNadia DianoCarla NicolucciSonia ErricoMarcello DallioAlessandro FedericoCarmelina LoguercioPaola StiusoPublished in: Scientific reports (2019)
Bisphenol A (BPA) and silybin are considered xenoestrogens and could interfere with the action of endogenous hormones. It was demonstrated a higher level of BPA in plasma of nonalcoholic steatohepatitis (NASH) patients, compared to those with steatosis (NAFL). We investigated the effect of BPA and silybin, alone or in combination, on proliferation, oxidative stress and steroid metabolism in HepG2 grown in high glucose concentration medium (H-HepG2). Cell viability was assessed by adding 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). TBARS were quantified by spectrophotometry. The effect of BPA, silybin and their combination on the expression of phosphorilized extracellular signal-regulated kinase (ERK), ERK and Caspase 3 was determined by Western blot analysis. The identifications of lipids and steroid hormones was performed by mass spectrometry. BPA elicited in H-HepG2 oxidative stress and steroid hormones oxidation leading to the formation of metabolite with estrogenic and genotoxic potentials. Silybin ameliorates the harmful BPA-induced effect decreasing glucose uptake and lipid peroxidation. Moreover silybin activates the synthesis of vitamin D3 metabolites and prevent the steroid hormones oxidation. BPA could be considered as an important risk factor in worsening and progression of NAFLD. At the same time silybin could be a valid support to counteract these effects in NASH patients.
Keyphrases
- high glucose
- cell proliferation
- oxidative stress
- end stage renal disease
- signaling pathway
- mass spectrometry
- ejection fraction
- newly diagnosed
- chronic kidney disease
- endothelial cells
- hydrogen peroxide
- prognostic factors
- dna damage
- pi k akt
- cell death
- blood pressure
- weight loss
- poor prognosis
- transcription factor
- tyrosine kinase
- nitric oxide
- bone marrow
- metabolic syndrome
- fatty acid
- cell therapy
- mouse model
- patient reported outcomes
- blood glucose
- simultaneous determination