Role of IL-17A receptor blocking in melanocyte survival: A strategic intervention against vitiligo.
Supriya BhardwajAlka BhatiaMuthu Sendhil KumaranDavinder ParsadPublished in: Experimental dermatology (2018)
Cytokines regulate immune response and inflammation and play an important role in depigmentation process of an autoimmune disease, vitiligo. We sought to determine how inflammatory cytokines influence the progression of vitiligo, and based on that, we develop a logical therapeutic intervention using primary melanocyte culture. Melanocytes were cultured and exposed to IL-17A, IL-1β, IFN-γ and TGF-β for 4 days. Melanocytes proliferation, tyrosinase assay and melanin content were measured. Real-Time PCR was used to analyse mRNA expression of genes specific for melanocytes growth and pigmentation. Anti-IL-17A receptor antibody was used to block IL-17A receptors expressed on melanocytes. Protein expression of MITF and TYR was assessed by immunofluorescence and Western blotting. A gradual decline in the melanocyte population, melanin content and tyrosinase activity was observed after different cytokine treatment. The expression of MITF and its downstream genes after blocking with anti-IL-17RA, an increased melanin content, increased expression of TYR, MITF along with its downstream genes, and cell proliferation was observed.
Keyphrases
- immune response
- cell proliferation
- randomized controlled trial
- poor prognosis
- genome wide
- oxidative stress
- binding protein
- multiple sclerosis
- dendritic cells
- signaling pathway
- rheumatoid arthritis
- dna methylation
- high throughput
- south africa
- cell cycle
- systemic sclerosis
- endothelial cells
- inflammatory response
- transforming growth factor
- toll like receptor
- epithelial mesenchymal transition
- transcription factor
- interstitial lung disease