Studying Antifatigue Mechanism of Tyr-Pro-Leu-Pro in Exercise Mice Using Label-Free Proteomics.
Jiaming CaiYe TaoLujuan XingJian ZhangZixu WangZihan ZhuWangang ZhangPublished in: Journal of agricultural and food chemistry (2024)
In our previous study, yeast-derived peptide Tyr-Pro-Leu-Pro (YPLP) was found to prolong treadmill time and relieve muscle fatigue in ICR mice. The present study aimed to further investigate the antifatigue mechanism of YPLP. Three doses of YPLP (10, 25, and 50 mg/kg·d) were given to exercise mice for 4 weeks. Results showed that YPLP reduced the oxidative response via the nuclear factor erythroid-2-related factor 2 (Nrf2) pathway and promoted energy metabolism through the AMP-activated protein kinase (AMPK) pathway. Label-free proteomics results showed that 81 differential abundance proteins (DAPs) were regulated by high-dose YPLP. These DAPs belonged to proteasome, mitochondrial, and muscle proteins. YPLP was mainly involved in proteasome, aminoacyl-tRNA biosynthesis, focal adhesion, and MAPK signal pathways to enhance muscle endurance. Furthermore, real-time quantitative PCR and Western blotting results proved that YPLP upregulated Psmd14 expression and downregulated p38 MAPK expression. Overall, this study revealed the mechanism behind YPLP to alleviate exercise fatigue.
Keyphrases
- label free
- skeletal muscle
- high intensity
- protein kinase
- high dose
- nuclear factor
- oxidative stress
- poor prognosis
- physical activity
- mass spectrometry
- low dose
- type diabetes
- toll like receptor
- high fat diet induced
- resistance training
- depressive symptoms
- microbial community
- cystic fibrosis
- inflammatory response
- high resolution
- south africa
- escherichia coli
- adipose tissue
- stem cell transplantation