Neglectibacter timonensis gen. nov., sp. nov. and Scatolibacter rhodanostii gen. nov., sp. nov., two anaerobic bacteria isolated from human stool samples.

Strains Marseille-P2265 T (=CSUR P2265 T  =DSM 102082  T ) and Marseille-P3890 T (=CSUR P3890 T  =CCUG 72341  T ) were isolated from stool samples using the culturomics approach. The 16S rRNA gene sequences of both strains were sequenced and compared by BLASTn to the NCBI database. Strains Marseille-P2265 T and Marseille-P3890 T were most closely related to Acutalibacter muris with identities of 94.3% and 91.5%, respectively. Between the two strains, the 16S rRNA gene sequence identity was 91.5%. Both strains are anaerobic Gram-positive, oxidase- and catalase-negative. The major fatty acid methyl esters (> 10%) in both strains are C 16:0 and anteiso-C 15:0 . Additionally, strain Marseille-P2265 T has iso-C 15:0 and C 14:0 , and strain Marseille-P3890 T , iso-C 14:0 . Strain Marseille-P2265 T has a genome size of 3,671,396-bp with a G + C content of 52.8%. As for strain Marseille-P3890 T , the genome is 2,702,024-bp-long with a 39.8% G + C content. The genomic comparison of closely related species with strains Marseille-P2265 T and Marseille-P3890 T showed that all digital DNA-DNA hybridization (dDDH), orthologous average nucleotide identity (OrthoANI) and average amino acid identity (AAI) values were lower than the published species thresholds (70% for dDDH, 95-96% for OrthoANI/AAI). Based on these results, it was concluded that strains Marseille-P2265 T and Marseille-P3890 T belong to two new genera in the family Oscillospiraceae. For these two genera, the names Neglectibacter gen. nov. and Scatolibacter gen. nov. were proposed, with strains Marseille-P2265 T and Marseille-P3890 T being the type strains of Neglectibacter timonensis gen. nov., sp. nov. and Scatolibacter rhodanostii gen. nov., sp. nov., respectively.