Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug-resistant Klebsiella pneumoniae and increasing the ability of macrophages.
Hui-Yun WangXiao-Chun ChenZhi-Han YanFan TuTian HeSubash C B GopinathXiao-Hong RuiFu-Tao CaoPublished in: Biotechnology and applied biochemistry (2021)
By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity-related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), and outer membrane protein expression were measured. Intranasal inoculation of pneumoconiosis was used to construct a mouse infection model, and the survival rate and cytokine expression level were tested. Human neutrophil peptide 1 (HNP-1) showed a significant antibacterial effect, which improved the permeability of the outer membrane of K. pneumoniae. Moreover, HNP-1 decreased LPS, CPS content, and outer membrane proteins. K. pneumoniae infection decreased antimicrobial peptide, oxidative stress, and autophagy-related genes, while HNP-1 increased these genes. After coculture with macrophages, the endocytosis of macrophages is enhanced and the bacterial load is greater in the K. pneumoniae + peptide group. Besides, higher levels of pp38 and pp65 in the K. pneumoniae + peptide group. HNP-1 rescued the cytotoxicity induced by K. pneumoniae. The survival rate is significantly improved after K. pneumoniae is treated by HNP-1. All cytokines in the peptide group were significantly higher. HNP-1 promotes immune sterilization by reducing the virulence of multidrug-resistant K. pneumoniae and increasing the ability of macrophages.
Keyphrases
- multidrug resistant
- klebsiella pneumoniae
- endothelial cells
- escherichia coli
- oxidative stress
- respiratory tract
- drug resistant
- pseudomonas aeruginosa
- poor prognosis
- inflammatory response
- staphylococcus aureus
- end stage renal disease
- newly diagnosed
- anti inflammatory
- cell death
- antimicrobial resistance
- dna methylation
- chronic kidney disease
- signaling pathway
- endoplasmic reticulum stress
- genome wide
- cell therapy
- toll like receptor
- gene expression
- biofilm formation
- bone marrow
- long non coding rna
- cystic fibrosis
- patient reported outcomes
- ischemia reperfusion injury
- peritoneal dialysis
- transcription factor
- free survival
- silver nanoparticles
- pluripotent stem cells
- lps induced
- wound healing