Detection of REST expression in the testis using epitope-tag knock-in mice generated by genome editing.
Ryuichi KimuraYukiko U InoueTakako KikkawaMisako TatehanaYuki MorimotoHitoshi InadaShinya OkiTakayoshi InoueNoriko OsumiPublished in: Developmental dynamics : an official publication of the American Association of Anatomists (2021)
We demonstrate that REST is expressed at the early step of spermatogenesis and suggest a possibility that REST may modulate the epigenetic state of male germline cells. Our KI mice may be useful for studying REST-associated molecular mechanisms of neurodevelopmental and age-related disorders.
Keyphrases
- genome editing
- crispr cas
- high fat diet induced
- induced apoptosis
- poor prognosis
- dna methylation
- gene expression
- squamous cell carcinoma
- oxidative stress
- adipose tissue
- cell proliferation
- dna damage
- long non coding rna
- metabolic syndrome
- skeletal muscle
- cell death
- endoplasmic reticulum stress
- quantum dots
- binding protein
- wild type