Login / Signup

Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

Peng CaiYunxia LiXiaoxin ZhaiLun YaoXiaojun MaLingyun JiaYongjin J Zhou
Published in: Bioresources and bioprocessing (2022)
α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.
Keyphrases
  • microbial community
  • carbon dioxide
  • recombinant human
  • fatty acid
  • poor prognosis
  • binding protein