Temporal and sequential transcriptional dynamics define lineage shifts in corticogenesis.
Tanzila MukhtarJeremie BredaManal A AdamMarcelo BoaretoPascal GrobeckerZahra KarimaddiniAlice GrisonKatja EschbachRamakrishnan ChandrasekharSwen VermeulMichal OkoniewskiMikhail PachkovCorey C HarwellSuzana AtanasoskiChristian BeiselDagmar IberErik van NimwegenVerdon TaylorPublished in: The EMBO journal (2022)
The cerebral cortex contains billions of neurons, and their disorganization or misspecification leads to neurodevelopmental disorders. Understanding how the plethora of projection neuron subtypes are generated by cortical neural stem cells (NSCs) is a major challenge. Here, we focused on elucidating the transcriptional landscape of murine embryonic NSCs, basal progenitors (BPs), and newborn neurons (NBNs) throughout cortical development. We uncover dynamic shifts in transcriptional space over time and heterogeneity within each progenitor population. We identified signature hallmarks of NSC, BP, and NBN clusters and predict active transcriptional nodes and networks that contribute to neural fate specification. We find that the expression of receptors, ligands, and downstream pathway components is highly dynamic over time and throughout the lineage implying differential responsiveness to signals. Thus, we provide an expansive compendium of gene expression during cortical development that will be an invaluable resource for studying neural developmental processes and neurodevelopmental disorders.
Keyphrases
- gene expression
- single cell
- transcription factor
- neural stem cells
- cell fate
- dna methylation
- heat shock
- spinal cord
- poor prognosis
- magnetic resonance imaging
- subarachnoid hemorrhage
- sentinel lymph node
- computed tomography
- oxidative stress
- early stage
- blood brain barrier
- congenital heart disease
- contrast enhanced
- dual energy