Proteomic Data in Morphine Addiction Versus Real Protein Activity: Metabolic Enzymes.
Anna AntolakAnna Bodzoń-KułakowskaEwa CetnarskaMonika PietruszkaMarta Marszałek-GrabskaJolanta KotlińskaPiotr SuderPublished in: Journal of cellular biochemistry (2017)
Drug dependence is an escalating problem worldwide and many efforts are being made to understand the molecular basis of addiction. The morphine model is widely used in these investigations. To date, at least 29 studies exploring the influence of morphine on mammals' proteomes have been published. Among various proteins indicated as up- or down-regulated, the expression changes of enzymes engaged in energy metabolism pathways have often been confirmed. To verify whether proteomics-indicated alterations in enzyme levels reflect changes in their activity, four enzymes: PK, MDH, Complex I, and Complex V were investigated in morphine addiction and abstinence models. After analyses of the rat brain mitochondria fraction in the model of morphine dependence, we found that one of the investigated enzymes (pyruvate kinase) showed statistically significant differences observed between morphine, control, and abstinence groups. J. Cell. Biochem. 118: 4323-4330, 2017. © 2017 Wiley Periodicals, Inc.
Keyphrases
- poor prognosis
- smoking cessation
- mass spectrometry
- single cell
- randomized controlled trial
- emergency department
- transcription factor
- systematic review
- cell death
- stem cells
- cell therapy
- mesenchymal stem cells
- atomic force microscopy
- machine learning
- tyrosine kinase
- protein protein
- reactive oxygen species
- adverse drug