ACE Inhibitory Peptides Derived from Muscovy Duck ( Cairina moschata ) Plasma.
Zongshuai ZhuHaoyu GuoYan XuAnthony Pius BasseyAhtisham AliMing HuangJichao HuangPublished in: Foods (Basel, Switzerland) (2022)
In this study, angiotensin-converting enzyme inhibitory peptides (ACE-IPs) derived from Muscovy duck ( Cairina moschata ) plasma hydrolysate (MDPH) were investigated. According to the general research protocol for bioactive peptides, the crude ACE-IPs of Muscovy duck plasma were separated and purified by ultrafiltration, gel chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC). Then the components with the highest ACE inhibition potential were selected for identification. Finally, the inhibition mechanism was explored by molecular docking and in silico simulated digestion. A total of 121 peptides was detected, and five were screened for synthesis verification and molecular docking. The peptide VALSSLRP revealed high ACE inhibitory activity (91.67 ± 0.73%) because this peptide bound tightly to the S1' pocket and formed 3 hydrogen bonds. Meaningfully, this work provides some new information about the generation of ACE-IPs derived from duck blood plasma.
Keyphrases
- angiotensin converting enzyme
- molecular docking
- angiotensin ii
- high performance liquid chromatography
- tandem mass spectrometry
- molecular dynamics simulations
- mass spectrometry
- simultaneous determination
- solid phase extraction
- amino acid
- randomized controlled trial
- healthcare
- liquid chromatography
- high resolution
- wound healing
- hyaluronic acid