CRISPR/Cas9-mediated genome editing directed by a 5S rRNA-tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens.

Chao FanWei ZhangXiaoyun SuWangli JiHuiying LuoYuhong ZhangBo LiuBin YaoHuoqing HuangXinxin Xu

Published in: Biotechnology for biofuels (2021)

The established CRISPR/Cas9 system is a powerful genetic operation tool for H. insolens, which will accelerate studies on the regulation mechanism of cellulase expression and engineering of H. insolens for higher cellulase production.

Keyphrases

crispr cas
genome editing
poor prognosis
dna methylation
genome wide
gene expression
transcription factor
anaerobic digestion
case control
copy number
binding protein