A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.

Elena RomitoIngrid BattistellaVera PlakhovaArteda PaplekajChiara ForastieriEmanuela ToffoloCarlo MusioLuciano ContiElena BattaglioliFrancesco Rusconi

Published in: Journal of neuroscience methods (2024)

Our protocol provides clear instructions for generating reliable human neuronal cultures with defined electrophysiological properties to investigate neuronal differentiation and model diseases in vitro.

Keyphrases

endothelial cells
randomized controlled trial
induced pluripotent stem cells
pluripotent stem cells
spinal cord
cerebral ischemia
spinal cord injury
subarachnoid hemorrhage