Effect of probiotic supplementation on oxidative stress markers in rats with diclofenac-induced hepatotoxicity.
Karima RianeMohamed SifourHouria Ouled-HaddarCristobal EspinosaMaria A EstebanMesbah LahouelPublished in: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2020)
In this study, we have investigated the effect of an antioxidant probiotic pretreatment toward an overdose of diclofenac in rats (100 mg/kg bw). Rats were treated daily with the probiotic Streptococcus salivarius St.sa (109 CFU) during seven successive days and then received a single treatment with diclofenac overdose in distilled water. Liver transaminases (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase), histology, glutathione (GSH) and malondialdehyde (MDA) level were investigated. In addition, both antioxidant enzyme activity and its mRNA gene expression were studied to evaluate diclofenac hepatotoxicity. The results indicated that probiotic pretreatment reduced diclofenac-induced hepatotoxicity through enhancement of the studied hepatic markers and regulation of antioxidant enzyme expression and activity. These findings indicate that the probiotic pretreatment protects rat liver against the oxidative stress induced by diclofenac overdose.
Keyphrases
- oxidative stress
- diabetic rats
- drug induced
- gene expression
- bacillus subtilis
- dna damage
- lactic acid
- high glucose
- induced apoptosis
- poor prognosis
- physical activity
- binding protein
- staphylococcus aureus
- signaling pathway
- candida albicans
- high resolution
- long non coding rna
- newly diagnosed
- heat shock
- replacement therapy
- stress induced