A novel methanol-free Pichia pastoris system for recombinant protein expression.
Wei ShenYing XueYiqi LiuChuixing KongXiaolong WangMengmeng HuangMenghao CaiXiangshan ZhouYuanxing ZhangMian ZhouPublished in: Microbial cell factories (2016)
We successfully constructed a novel methanol-free expression system Δdak-DHA. This modified expression platform preserved the favorable regulatable nature of P AOX1 , providing a potential alternative to the traditional system.