Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton.

Mohamed RamadanMuna AlariqiYizan MaYanlong LiZhenping LiuRui ZhangShuangxia JinLing MinXianlong Zhang

Published in: Plant methods (2021)

All targeted genes were successfully edited with high specificity. Our pooled sgRNAs assembly offers a simple, fast and efficient method/strategy to target multiple genes in one time and surely accelerated the study of genes function in cotton.

Keyphrases

crispr cas
genome wide
bioinformatics analysis
genome wide identification
genome editing
dna methylation
randomized controlled trial
clinical trial
gene expression
transcription factor
structural basis