Combinatorial genetics in liver repopulation and carcinogenesis with a in vivo CRISPR activation platform.

Kirk J WangensteenYue J WangZhixun DouAmber W WangElham Mosleh-ShiraziMax A HorlbeckLuke A GilbertJonathan S WeissmanShelley L BergerKlaus H Kaestner

Published in: Hepatology (Baltimore, Md.) (2018)

The in vivo CRISPRa platform developed here allows for parallel and combinatorial genetic screens in live animals; this approach enables screening for drivers and suppressors of cell replication and tumor initiation. (Hepatology 2017).

Keyphrases

genome wide
high throughput
single cell
dna methylation
crispr cas
copy number
cell therapy
genome editing
mesenchymal stem cells