Comparison of Cellular Target Engagement Methods for the Tubulin Deacetylases Sirt2 and HDAC6: NanoBRET, CETSA, Tubulin Acetylation, and PROTACs.

Anja VogelmannManfred Jung Finn K Hansen Matthias Schiedel

Published in: ACS pharmacology & translational science (2022)

The tubulin deacetylases Sirt2 and HDAC6 have been associated with the development of various diseases. Herein, we discuss recent approaches that enable cellular target engagement studies for these deacetylases and thus play a critical role in the evaluation of small molecule inhibitors of Sirt2 or HDAC6 as potential therapeutic agents.

Keyphrases

histone deacetylase
small molecule
oxidative stress
ischemia reperfusion injury
social media
protein protein
clinical evaluation