Potential Anti-inflammatory Effects of the Fruits of Paulownia tomentosa.
Hyung-Won RyuYhun Jung ParkSu Ui LeeSeoghyun LeeHeung Joo YukKyeong-Hwa SeoYeah-Un KimBang Yeon HwangSei-Ryang OhPublished in: Journal of natural products (2017)
As part of an ongoing search for new natural products from medicinal plants to treat respiratory disease, six new compounds, a dihydroflavonol (1) and five C-geranylated flavanones (3, 6, 8, 13, and 14), and 13 known compounds were isolated from mature fruits of Paulownia tomentosa. The structures of the new compounds were determined via interpretation of their spectroscopic data (1D and 2D NMR, UV, IR, ECD, and MS). In biological activity assays with human alveolar basal epithelial cells, the expression of TNF-α-induced proinflammatory cytokines (IL-8 and IL-6) was reduced significantly by the EtOAc fraction of a P. tomentosa extract as well as by the new compounds isolated from this fraction. Furthermore, the majority of the isolates (1-19 except 5-7) were found to inhibit human neutrophil elastase (HNE) activity, with IC50 values ranging from 2.4 ± 1.0 to 74.7 ± 8.5 μM. In kinetic enzymatic assays with the HNE substrate MeOSuc-AAPV-pNA, compound 17 exhibited the highest inhibitory activity (Ki = 3.2 μM) via noncompetitive inhibition. These findings suggest that the flavanone constituents of P. tomentosa fruits may be valuable for the development of new drug candidates to treat airway inflammation.
Keyphrases
- endothelial cells
- high glucose
- induced pluripotent stem cells
- high resolution
- high throughput
- poor prognosis
- multiple sclerosis
- magnetic resonance
- pluripotent stem cells
- oxidative stress
- mass spectrometry
- neoadjuvant chemotherapy
- big data
- squamous cell carcinoma
- emergency department
- hydrogen peroxide
- diabetic rats
- nitric oxide
- molecular docking
- machine learning
- lymph node
- long non coding rna
- genetic diversity
- artificial intelligence
- molecular dynamics simulations
- solid state