Cell to rodent: toxicological profiling of folate grafted thiomer enveloped nanoliposomes.
Muhammad Farhan SohailHafiz Shoaib SarwarIbrahim JavedAkhtar NadhmanSyed Zajif HussainHamid SaeedAbida RazaNadeem Irfan BukhariIrshad HussainGul ShahnazPublished in: Toxicology research (2017)
Polymeric nanomaterials, hybridized with lipid components, e.g. phosphocholine or fatty acids, are currently being explored for efficient nano-platforms for hydrophobic drugs. However, their toxicology and toxicokinetics need to be established before enabling their clinical potential. The aim of this study was to investigate the toxicological profile of thiomer enveloped hybrid nanoliposomes (ENLs) and bare nanoliposomes (NLs), loaded with docetaxel (DTX) hydrophobic drug, biocompatible nano-carriers for therapeutic cargo. The in vitro toxicity of hybrid ENLs and NLs was evaluated towards the HCT-116 colon cancer cell line. Biocompatibility was explored against macrophages and acute oral toxicity was examined in mice for 14 days. The anticancer IC50 for ENLs was 0.148 μg ml-1 compared with 2.38 μg ml-1 for pure docetaxel (DTX). The human macrophage viability remained above 65% and demonstrated a high level of biocompatibility and safety of ENLs. In vivo acute oral toxicity showed slight changes in serum biochemistry and haematology but no significant toxicities were observed referring to the safety of DTX loaded hybrid ENLs. On histological examination, no lesions were determined on the liver, heart and kidney. These studies showed that hybrid ENLs can serve as a safe and biocompatible platform for oral delivery of hydrophobic drugs.
Keyphrases
- ionic liquid
- drug delivery
- drug induced
- liver failure
- fatty acid
- oxidative stress
- drug release
- cancer therapy
- respiratory failure
- single cell
- oxide nanoparticles
- endothelial cells
- aortic dissection
- adipose tissue
- metabolic syndrome
- wound healing
- cell therapy
- high throughput
- type diabetes
- mesenchymal stem cells
- emergency department
- squamous cell carcinoma
- atrial fibrillation
- intensive care unit
- human health
- adverse drug
- cell death
- bone marrow
- risk assessment
- induced pluripotent stem cells
- pluripotent stem cells
- mechanical ventilation