Milk lactoperoxidase decreases ID1 and ID3 expression in human oral squamous cell carcinoma cell lines.
Layla PanahipourMaria De BiasiTheresa Sophia BokorAlexandra ThajerNadja HaidenReinhard GruberPublished in: Scientific reports (2020)
Milk consumption may modify the risk of squamous cell carcinoma. The role of milk to modulate the gene expression in oral squamous cell carcinoma cells has not been investigated so far. Here, HSC2 oral squamous carcinoma cells were exposed to an aqueous fraction of human milk and a whole-genome array was performed. Among the genes that were significantly reduced by human and cow milk were the DNA-binding protein inhibitor 1 (ID1), ID3 and Distal-Less Homeobox 2 (DLX2) in HSC2 cells. Also, in TR146 oral squamous carcinoma cells, there was a tendency towards a decreased gene expression. Upon size fractionation, lactoperoxidase but not lactoferrin and osteopontin was identified to reduce ID1 and ID3 in HSC2 cells. Dairy products and hypoallergenic infant formula failed to decrease the respective genes. These data suggest that milk can reduce the expression of transcription factors in oral squamous carcinoma cells.
Keyphrases
- gene expression
- human milk
- binding protein
- induced apoptosis
- squamous cell carcinoma
- endothelial cells
- high grade
- poor prognosis
- cell cycle arrest
- squamous cell
- dna methylation
- low grade
- genome wide
- transcription factor
- induced pluripotent stem cells
- low birth weight
- high resolution
- pluripotent stem cells
- endoplasmic reticulum stress
- mass spectrometry
- radiation therapy
- single molecule
- electronic health record
- minimally invasive
- big data
- long non coding rna
- machine learning
- cell proliferation
- circulating tumor
- signaling pathway
- rectal cancer
- pi k akt