The Histone Variant H3.3 Is Required for Plant Growth and Fertility in Arabidopsis .
Xiaogang LongWandong YangYanfang LvXiaoming ZhongLin ChenQingzhu LiZhaopeng LvYanzhuo LiYajun CaiHongchun YangPublished in: International journal of molecular sciences (2024)
Histones are the core components of the eukaryote chromosome, and have been implicated in transcriptional gene regulation. There are three major isoforms of histone H3 in Arabidopsis . Studies have shown that the H3.3 variant is pivotal in modulating nucleosome structure and gene transcription. However, the function of H3.3 during development remains to be further investigated in plants. In this study, we disrupted all three H3.3 genes in Arabidopsis . Two triple mutants, h3.3cr-4 and h3.3cr-5, were created by the CRISPR/Cas9 system. The mutant plants displayed smaller rosettes and decreased fertility. The stunted growth of h3.3cr-4 may result from reduced expression of cell cycle regulators. The shorter stamen filaments, but not the fertile ability of the gametophytes, resulted in reduced fertility of h3.3cr-4 . The transcriptome analysis suggested that the reduced filament elongation of h3.3cr-4 was probably caused by the ectopic expression of several JASMONATE-ZIM DOMAIN ( JAZ ) genes, which are the key repressors of the signaling pathway of the phytohormone jasmonic acid (JA). These observations suggest that the histone variant H3.3 promotes plant growth, including rosette growth and filament elongation.
Keyphrases
- plant growth
- transcription factor
- cell cycle
- genome wide identification
- signaling pathway
- crispr cas
- poor prognosis
- genome wide
- dna methylation
- cell proliferation
- gene expression
- copy number
- genome editing
- binding protein
- long non coding rna
- pi k akt
- high resolution
- wild type
- genome wide analysis
- heat stress
- endoplasmic reticulum stress
- bioinformatics analysis
- induced apoptosis
- heat shock protein