Evaluation of the Antioxidant Activity of Nigella sativa L. and Allium ursinum Extracts in a Cellular Model of Doxorubicin-Induced Cardiotoxicity.
Raluca-Maria PopIoana Corina BocsanAnca Dana BuzoianuVeronica Sanda ChedeaSonia-Ancuța SocaciMichela PecoraroAda PopoloPublished in: Molecules (Basel, Switzerland) (2020)
Natural products black cumin-Nigella sativa (N. sativa) and wild garlic-Allium ursinum (AU) are known for their potential role in reducing cardiovascular risk factors, including antracycline chemotherapy. Therefore, this study investigates the effect of N. sativa and AU water and methanolic extracts in a cellular model of doxorubicin (doxo)-induced cardiotoxicity. The extracts were characterized using Ultraviolet-visible (UV-VIS) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, Liquid Chromatography coupled with Mass Spectrometry (LC-MS) and Gas Chromatography coupled with Mass Spectrometry (GC-MS) techniques. Antioxidant activity was evaluated on H9c2 cells. Cytosolic and mitochondrial reactive oxygen species (ROS) release was evaluated using 2',7'-dichlorofluorescin-diacetate (DHCF-DA) and mitochondria-targeted superoxide indicator (MitoSOX red), respectively. Mitochondrial membrane depolarization was evaluated by flow cytometry. LC-MS analysis identified 12 and 10 phenolic compounds in NSS and AU extracts, respectively, with flavonols as predominant compounds. FT-IR analysis identified the presence of carbohydrates, amino acids and lipids in both plants. GC-MS identified the sulfur compounds in the AU water extract. N. sativa seeds (NSS) methanolic extract had the highest antioxidant activity reducing both intracellular and mitochondrial ROS release. All extracts (excepting AU methanolic extract) preserved H9c2 cells viability. None of the investigated plants affected the mitochondrial membrane depolarization. N. sativa and AU are important sources of bioactive compounds with increased antioxidant activities, requiring different extraction solvents to obtain the pharmacological effects.
Keyphrases
- oxidative stress
- mass spectrometry
- reactive oxygen species
- induced apoptosis
- liquid chromatography
- gas chromatography
- sensitive detection
- diabetic rats
- reduced graphene oxide
- tandem mass spectrometry
- cardiovascular risk factors
- high resolution mass spectrometry
- high resolution
- dna damage
- cancer therapy
- flow cytometry
- high performance liquid chromatography
- cell death
- high glucose
- anti inflammatory
- capillary electrophoresis
- drug delivery
- visible light
- metabolic syndrome
- cardiovascular disease
- single molecule
- gold nanoparticles
- signaling pathway
- solid phase extraction
- endoplasmic reticulum stress
- drinking water
- cell proliferation
- ionic liquid
- endothelial cells
- hydrogen peroxide
- genetic diversity
- rectal cancer
- solid state
- ms ms
- fatty acid