Protein expression and purification

Keyphrases

Publications

volume 225, 2024

Tetsuya Okuda, Katsuya Kato
A simple procedure for preparing biotinylated immunoglobulin M from hybridoma culture medium. Protein expression and purification 225 (2024)
Yong Fu, Yangguang Xu, Maodan Zhang, Fengjuan Lv
Removal of signal peptide variants by cation exchange chromatography: A case study. Protein expression and purification 225 (2024)
Dylan Moodley, Angela Botes
A carboxymethyl cellulase from the yeast Cryptococcus gattii WM276: Expression, purification and characterisation. Protein expression and purification 225 (2024)
Wei Chen, Ting Zhang, Peter K Wang, Chien-Chun Liao, Yifeng Li, Yan Wan
Partition coefficient screening - An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case. Protein expression and purification 225 (2024)
Nurul Nadia Mohamad Alias, Eugene Boon Beng Ong, Mervyn W O Liew
Removal and monitoring of residual nucleic acids from core streptavidin inclusion bodies for increased refolding yield. Protein expression and purification 225 (2024)
Alexsandra Nascimento Ferreira, Tatielle Pereira Silva, Ciro Ramon Félix, Julia Lins Lopes, Cláudio Wiliam Victor Dos Santos, Dávida Maria Ribeiro Cardoso Dos Santos, Melissa Fontes Landell, Francis Soares Gomes, Hugo Juarez Vieira Pereira
Use of waste frying oil and coconut pulp for the production, isolation, and characterization of a new lipase from Moesziomyces aphidis. Protein expression and purification 225 (2024)
Simon Messing, Stephanie R T Widmeyer, John-Paul Denson, Jennifer Mehalko, Vanessa E Wall, Matthew Drew, Kelly Snead, Min Hong, Carissa Grose, Dominic Esposito, William Gillette
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase. Protein expression and purification 225 (2024)

volume 224, 2024

Si-Yuan Su, Yong-Shan Zheng, Hui Mao, Li-Bing Zhao, Man-Yi Zhu, Yu-Feng Yang, Ling-Ting Li, Zi-Ru Wang, Cheng He
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli. Protein expression and purification 224 (2024)
Weitao Wang, Yiwei Meng, Xin Yin, Peipei Zhao, Mengmeng Wang, Jingli Ren, Jiyuan Zhang, Lixin Zhang, Yunqian Cui, Xuekui Xia
Novel heterologously expressed protein, AjPSPLP-3, derived from Apostichopus japonicus exhibits cell proliferation and migration activities. Protein expression and purification 224 (2024)
Hongyu Yuan, Sai Vivek Prabhala, Michael J Coolbaugh, Samuel D Stimple, David W Wood
Improved self-cleaving precipitation tags for efficient column free bioseparations. Protein expression and purification 224 (2024)
Weitao Wang, Yiwei Meng, Xin Yin, Peipei Zhao, Mengmeng Wang, Jingli Ren, Jiyuan Zhang, Lixin Zhang, Yunqian Cui, Xuekui Xia
Novel heterologously expressed protein, AjPSPLP-3, derived from Apostichopus japonicus exhibits cell proliferation and migration activities. Protein expression and purification 224 (2024)
Petra Riegerová, Matej Horváth, Filip Šebesta, Jan Sýkora, Miroslav Šulc, Antonín Vlček
Single-step purification and characterization of Pseudomonas aeruginosa azurin. Protein expression and purification 224 (2024)
Yanxin Wang, Tingting Xie, Chenlong Ma, Yujie Zhao, Jingchen Li, Zhendong Li, Xianfeng Ye
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659. Protein expression and purification 224 (2024)
Si-Yuan Su, Yong-Shan Zheng, Hui Mao, Li-Bing Zhao, Man-Yi Zhu, Yu-Feng Yang, Ling-Ting Li, Zi-Ru Wang, Cheng He
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli. Protein expression and purification 224 (2024)
Ze Li, Su-Yan Tian
A new alkaline pectin lyase with novel thermal and pH stability from Bacilus velezensis. Protein expression and purification 224 (2024)
Qingsong Zeng, Peifang Lai, Mingqin Huang, Ximing Peng, Junjie Huang, Qintao Chen, Yanxu Chen, Huaqian Wang
MAM7 from Vibrio parahaemolyticus: Expression, purification and effects on RAW264.7 cells. Protein expression and purification 224 (2024)
Petra Riegerová, Matej Horváth, Filip Šebesta, Jan Sýkora, Miroslav Šulc, Antonín Vlček
Single-step purification and characterization of Pseudomonas aeruginosa azurin. Protein expression and purification 224 (2024)
Hongyu Yuan, Sai Vivek Prabhala, Michael J Coolbaugh, Samuel D Stimple, David W Wood
Improved self-cleaving precipitation tags for efficient column free bioseparations. Protein expression and purification 224 (2024)
Robert Eves, Peter L Davies
Protein inclusion into ice can dissociate subunits. Protein expression and purification 224 (2024)
Qingsong Zeng, Peifang Lai, Mingqin Huang, Ximing Peng, Junjie Huang, Qintao Chen, Yanxu Chen, Huaqian Wang
MAM7 from Vibrio parahaemolyticus: Expression, purification and effects on RAW264.7 cells. Protein expression and purification 224 (2024)
Constantinos Chatzicharalampous, Herwig Schüler
A multidomain PARP14 construct suitable for bacterial expression. Protein expression and purification 224 (2024)
Constantinos Chatzicharalampous, Herwig Schüler
A multidomain PARP14 construct suitable for bacterial expression. Protein expression and purification 224 (2024)
Qingsong Zeng, Peifang Lai, Mingqin Huang, Ximing Peng, Junjie Huang, Qintao Chen, Yanxu Chen, Huaqian Wang
MAM7 from Vibrio parahaemolyticus: Expression, purification and effects on RAW264.7 cells. Protein expression and purification 224 (2024)
Weitao Wang, Yiwei Meng, Xin Yin, Peipei Zhao, Mengmeng Wang, Jingli Ren, Jiyuan Zhang, Lixin Zhang, Yunqian Cui, Xuekui Xia
Novel heterologously expressed protein, AjPSPLP-3, derived from Apostichopus japonicus exhibits cell proliferation and migration activities. Protein expression and purification 224 (2024)
Petra Riegerová, Matej Horváth, Filip Šebesta, Jan Sýkora, Miroslav Šulc, Antonín Vlček
Single-step purification and characterization of Pseudomonas aeruginosa azurin. Protein expression and purification 224 (2024)
Si-Yuan Su, Yong-Shan Zheng, Hui Mao, Li-Bing Zhao, Man-Yi Zhu, Yu-Feng Yang, Ling-Ting Li, Zi-Ru Wang, Cheng He
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli. Protein expression and purification 224 (2024)
Yanxin Wang, Tingting Xie, Chenlong Ma, Yujie Zhao, Jingchen Li, Zhendong Li, Xianfeng Ye
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659. Protein expression and purification 224 (2024)
Robert Eves, Peter L Davies
Protein inclusion into ice can dissociate subunits. Protein expression and purification 224 (2024)
Ze Li, Su-Yan Tian
A new alkaline pectin lyase with novel thermal and pH stability from Bacilus velezensis. Protein expression and purification 224 (2024)
Yanxin Wang, Tingting Xie, Chenlong Ma, Yujie Zhao, Jingchen Li, Zhendong Li, Xianfeng Ye
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659. Protein expression and purification 224 (2024)
Constantinos Chatzicharalampous, Herwig Schüler
A multidomain PARP14 construct suitable for bacterial expression. Protein expression and purification 224 (2024)

volume 223, 2024

Feng Liu, Song Chen, Xingbang Chen, Bin Yong, Bing He
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum. Protein expression and purification 223 (2024)
Hemant Kumar, Aayushi Bajaj, Paras Kumar, Rupesh Aggarwal, Vinayak Chalia, Rajesh Kumar Pradhan, Ritu Yadav, Shalini Sinha, Vishad Agarwal, William Harries, Meenakshi Dua, Robert M Stroud, Atul Kumar Johri
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica. Protein expression and purification 223 (2024)
Daichi Morimoto, Erik Walinda
A fast and simple automated multi-step protein purification method for ÄKTA go systems. Protein expression and purification 223 (2024)
Xiuli Cui, Zheng Fu, Hainan Wang, Wengong Yu, Feng Han
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99. Protein expression and purification 223 (2024)
Yi-Xuan Zhao, Xiao Xiang, Xi-Peng Liu
The biochemical characterization of a TatD nuclease from Thermus thermophilus. Protein expression and purification 223 (2024)
Feng Liu, Song Chen, Xingbang Chen, Bin Yong, Bing He
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum. Protein expression and purification 223 (2024)
Yi-Xuan Zhao, Xiao Xiang, Xi-Peng Liu
The biochemical characterization of a TatD nuclease from Thermus thermophilus. Protein expression and purification 223 (2024)
Pingping Meng, Liyan Zhu, Jiatong Guo, Yuanyuan Li, Yu Wei, Jiawei Sun, Jingling Zhu
Preparation of recombinant neuritin protein. Protein expression and purification 223 (2024)
Daichi Morimoto, Erik Walinda
A fast and simple automated multi-step protein purification method for ÄKTA go systems. Protein expression and purification 223 (2024)
Shuyan Duan, Yaoyao Wu, Tianzhu Chao, Nan Zhang, Zhaoyi Wei, Rui Ji
Improving the catalytic activity and thermostability of Aspergillus niger xylanase through computational design. Protein expression and purification 223 (2024)
Feng Liu, Song Chen, Xingbang Chen, Bin Yong, Bing He
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum. Protein expression and purification 223 (2024)
Shuzhen Li, Wei Shen, Yuanyuan Xia, Xianzhong Chen, Haiquan Yang
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose. Protein expression and purification 223 (2024)
Kimia Ahadi-Amandi, Seyyed Abolghasem Ghadami, Narges Sayari, Reza Khodarahmi
Purification of α-lactalbumin and β-lactoglobulin from cow milk. Protein expression and purification 223 (2024)
Yi-Xuan Zhao, Xiao Xiang, Xi-Peng Liu
The biochemical characterization of a TatD nuclease from Thermus thermophilus. Protein expression and purification 223 (2024)
Pingping Meng, Liyan Zhu, Jiatong Guo, Yuanyuan Li, Yu Wei, Jiawei Sun, Jingling Zhu
Preparation of recombinant neuritin protein. Protein expression and purification 223 (2024)
Jk Nithya Shree, T Premika, S Sharlin, A Annie Aglin
Diverse approaches to express recombinant spike protein: A comprehensive review. Protein expression and purification 223 (2024)
Xiuli Cui, Zheng Fu, Hainan Wang, Wengong Yu, Feng Han
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99. Protein expression and purification 223 (2024)
Xiuli Cui, Zheng Fu, Hainan Wang, Wengong Yu, Feng Han
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99. Protein expression and purification 223 (2024)
Shuzhen Li, Wei Shen, Yuanyuan Xia, Xianzhong Chen, Haiquan Yang
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose. Protein expression and purification 223 (2024)