Blueberry Polyphenols Ameliorate Visible Light and Lipid-Induced Injury of Retinal Pigment Epithelial Cells.
Yi-Xiang LiuMing LiuQingchou ChenGuang-Ming LiuMin-Jie CaoLechang SunZhenhua LuCaihua GuoPublished in: Journal of agricultural and food chemistry (2018)
Although dietary polyphenols are known to be beneficial to vision, the protective distinctions among different types of polyphenols are unclear. In this work, the visual benefits of various blueberry polyphenols were evaluated using an in vitro model of visible light-lipid-induced injury of retinal pigment epithelial cells. Results showed that, at 10.0 μg/mL, the phenolic acid-rich fraction was superior in inhibiting cell death (93.6% ± 2.8% of cell viability). Anthocyanin- and flavonoid-rich fractions shared similar advantages in preventing the expression of senescence-associated β-galactosidase (34.8% ± 11.1% and 32.2% ± 9.7% of aged cells, respectively) and overexpression of vascular endothelial growth factor (51.8 ± 3.5 and 54.1 ± 6.5 pg/mL, respectively). The flavonoid-rich fraction also showed high activity in ameliorating phagocytosis (70.3% ± 12.6%) and cellular oxidative stress. These results were further confirmed by using the corresponding polyphenol standards. Improved inhibitory effects of polyphenol mixture on cell death and senescence-associated β-galactosidase expression were also observed. Therefore, various polyphenols play diverse roles and exert synergistic effects in nourishing the retina.
Keyphrases
- visible light
- cell death
- vascular endothelial growth factor
- cell cycle arrest
- diabetic rats
- endothelial cells
- oxidative stress
- high glucose
- poor prognosis
- induced apoptosis
- dna damage
- drug induced
- signaling pathway
- fatty acid
- transcription factor
- binding protein
- endoplasmic reticulum stress
- ischemia reperfusion injury