Discovery Proteomics of Human Placental Tissue.
Allyson L MellingerKrista McCoyDuy An T MiniorTaufika Islam WilliamsPublished in: Rapid communications in mass spectrometry : RCM (2021)
We describe a label-free proteomics protocol for the interrogation of the placental proteome. Step-by-step directions are provided from tissue clean-up and preparation, proteolytic digestion, nanoLC-MS/MS data collection and data analysis. The workflow has been applied towards exploring differential protein expression patterns in placentas from women who have been exposed to drugs during pregnancy relative to those who have not. We collected twenty tissue specimens, each representing a combination of spatially diverse sections across the placenta. These specimens were analyzed in the work described here, to survey information across the entire organ. This protocol can be scaled up or down as needed.
Keyphrases
- label free
- data analysis
- ms ms
- randomized controlled trial
- mass spectrometry
- electronic health record
- endothelial cells
- polycystic ovary syndrome
- small molecule
- high throughput
- cross sectional
- adipose tissue
- machine learning
- big data
- induced pluripotent stem cells
- fine needle aspiration
- pluripotent stem cells
- molecularly imprinted
- simultaneous determination
- ultra high performance liquid chromatography