Red-Fleshed Apples Rich in Anthocyanins and White-Fleshed Apples Modulate the Aorta and Heart Proteome in Hypercholesterolaemic Rats: The AppleCOR Study.
Úrsula CatalánAnna PedretSilvia YusteLaura RubióCarme PiñolBerner Andrée Sandoval-RamírezJudit CompanysElisabet FoguetPol HerreroNuria CanelaMaria-José MotilvaRosa SolàPublished in: Nutrients (2022)
The impact of a red-fleshed apple (RFA) rich in anthocyanins (ACNs), a white-fleshed apple (WFA) without ACNs, and an extract infusion from Aronia fruit (AI) equivalent in dose of cyanidin-3-O-galactoside (main ACN) as RFA was determined by the proteome profile of aorta and heart as key cardiovascular tissues. Hypercholesterolaemic Wistar rats were separated into six groups ( n = 6/group; three males and three females) and the proteomic profiles were analyzed using nanoliquid chromatography coupled to mass spectrometry. No adverse events were reported and all products were well tolerated. RFA downregulated C1QB and CFP in aorta and CRP in heart. WFA downregulated C1QB and CFP in aorta and C9 and C3 in aorta and heart, among other proteins. AI downregulated PRKACA, IQGAP1, and HSP90AB1 related to cellular signaling. Thus, both apples showed an anti-inflammatory effect through the complement system, while RFA reduced CRP. Regardless of the ACN content, an apple matrix effect was observed that involved different bioactive components, and inflammatory proteins were reduced.
Keyphrases
- aortic valve
- pulmonary artery
- radiofrequency ablation
- mass spectrometry
- heart failure
- anti inflammatory
- atrial fibrillation
- artificial intelligence
- coronary artery
- oxidative stress
- pulmonary hypertension
- liquid chromatography
- low dose
- gene expression
- aortic dissection
- heat shock protein
- machine learning
- high speed
- ms ms