A comparative study on the allergenic potential of β-lactoglobulin conjugated to glucose, caffeic acid and caffeoyl glucopyranose.
Yang WangKai ZhangYi-Ying MaoYan-Hong ShaoJun LiuZong Cai TuPublished in: Food & function (2023)
This work intends to perform a comparative study on the allergenic potential of β-lactoglobulin (BLG)-glucose, BLG-caffeic acid and BLG-caffeoyl glucopyranose conjugates. The modifications changed the molecular weight and multi-structure of BLG and destroyed the allergenic epitope, which resulted in a decrease in the IgE binding level and the release ability of histamine and IL-6 in KU812 cells. Compared with BLG, the conjugates reduced the serum levels of IgG, IgE, β-Hex and IL-4 in vivo , while increasing the level of interferon-γ, which caused an imbalance of Th1/Th2 immune response. Meanwhile, these conjugates not only increased the relative abundance of allergy-related gut flora, such as Lachnospiraceae, norank_o_Clostridia_UCG-014, Erysipelotrichaceae, Turicibacter and Lachnospiraceae_NK4A136_group , but also improved the level of short-chain fatty acids (SCFAs). Caffeoyl glucopyranose with a large molecular weight and long carbon chains exerted a great influence on the allergy-related gut flora and SCFAs. Therefore, the changes in the Th1/Th2 balance and SCFA level produced by the allergy-related gut flora were responsible for reducing the potential allergy of BLG.
Keyphrases
- immune response
- fatty acid
- induced apoptosis
- cancer therapy
- atopic dermatitis
- dendritic cells
- blood glucose
- type diabetes
- infectious diseases
- risk assessment
- human health
- oxidative stress
- toll like receptor
- blood pressure
- drug delivery
- monoclonal antibody
- dna binding
- binding protein
- cell cycle arrest
- wastewater treatment