CRISPR-Cas9-mediated knockout of SPRY2 in human hepatocytes leads to increased glucose uptake and lipid droplet accumulation.

Naomi L CookMilos PjanicAndrew G EmmerichAbhiram S RaoSusanne HettyJoshua W KnowlesThomas QuertermousCasimiro Castillejo-LópezErik Ingelsson

Published in: BMC endocrine disorders (2019)

These findings suggest a role for SPRY2 in glucose and lipid metabolism in hepatocytes and contribute to clarifying the function of this gene in the context of metabolic diseases.

Keyphrases

crispr cas
genome editing
endothelial cells
blood glucose
liver injury
fatty acid
induced pluripotent stem cells
genome wide
high throughput
single cell
copy number
drug induced
pluripotent stem cells
type diabetes
gene expression
dna methylation
genome wide identification
insulin resistance
glycemic control