Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex.
Brian R LeeRachel DalleyJeremy A MillerThomas ChartrandJennie CloseRusty MannMukora AliceLindsay NgLauren AlfilerKatherine BakerDarren BertagnolliKrissy BrounerTamara CasperEva Adrienn CsajbokNicholas DonadioStan L W DriessensTom EgdorfRachel EnstromAnna A GalakhovaAmanda GaryEmily GelfandJeff GoldyKristen HadleyTim S HeistekDiJon HillWen-Hsien HouNelson JohansenNikolas L JorstadLisa KimAgnes Katalin KocsisLauren KruseMichael KunstGabriela LeónBrian R LongMatthew MalloryMichelle MaxwellMedea McGrawDelissa A McMillenErica J MeliefGábor MolnárMarty T MortrudDakota NewmanJulie NyhusXimena Opitz-ArayaAttila OzsvárTrangthanh PhamChristina Alice PomLydia PotekhinaRamkumar RajanbabuAugustin RuizSusan M SunkinIldikó SzötsNaz TaskinBargavi ThyagarajanMichael TieuJessica TrinhSara VargasDavid VumbacoFemke WaleboerSarah Walling-BellNatalie WeedGrace WilliamsJulia WilsonShenqin YaoThomas ZhouPál BarzóTrygve E BakkenCharles CobbsNick DeeRichard G EllenbogenLuke EspositoManuel FerreiraNathan W GouwensBenjamin L GrannanRyder P GwinnJason S HauptmanRebecca D HodgeTim JarskyC Dirk KeeneAndrew L KoAnders Rosendal KorshoejBoaz P LeviKaare MeierJeffrey G OjemannAnoop PatelJacob RuzevickDaniel L SilbergeldKimberly A SmithJens Christian Hedemann SørensenJack WatersHongkui ZengJim BergMarco CapognaNatalia A GoriounovaBrian E KalmbachChristiaan P J de KockHuibert D MansvelderStaci A SorensenGabor TamasEd S LeinJonathan T TingPublished in: Science (New York, N.Y.) (2023)
Human cortex transcriptomic studies have revealed a hierarchical organization of γ-aminobutyric acid-producing (GABAergic) neurons from subclasses to a high diversity of more granular types. Rapid GABAergic neuron viral genetic labeling plus Patch-seq (patch-clamp electrophysiology plus single-cell RNA sequencing) sampling in human brain slices was used to reliably target and analyze GABAergic neuron subclasses and individual transcriptomic types. This characterization elucidated transitions between PVALB and SST subclasses, revealed morphological heterogeneity within an abundant transcriptomic type, identified multiple spatially distinct types of the primate-specialized double bouquet cells (DBCs), and shed light on cellular differences between homologous mouse and human neocortical GABAergic neuron types. These results highlight the importance of multimodal phenotypic characterization for refinement of emerging transcriptomic cell type taxonomies and for understanding conserved and specialized cellular properties of human brain cell types.
Keyphrases
- single cell
- rna seq
- endothelial cells
- high throughput
- induced pluripotent stem cells
- pluripotent stem cells
- induced apoptosis
- sars cov
- stem cells
- gene expression
- genome wide
- dna repair
- spinal cord injury
- mesenchymal stem cells
- cell proliferation
- pain management
- case control
- bone marrow
- functional connectivity
- cell cycle arrest