Antimicrobial activity against Staphylococcus aureus and genome features of Lactiplantibacillus plantarum LR-14 from Sichuan pickles.
Shuhui YangLei LiuJingwen WangShuyu GuoGuorong LiuXing ChenXi DengMingxia TuYufei TaoYu RaoPublished in: Archives of microbiology (2022)
The persistence of Staphylococcus aureus within biofilm can lead to contamination of medical devices and life-threatening infections. Luckily, lactic acid bacteria (LAB) have an inhibitory effect on the growth of these bacteria. This study aims to select LAB strains from fermented vegetables, and analyze their potential inhibition activities against S. aureus. In total, 45 isolates of LAB were successfully isolated from Sichuan pickles, and the CFS of Lactiplantibacillus plantarum LR-14 exerted the strongest inhibitory effect against S. aureus. Moreover, S. aureus cells in planktonic and biofilm states both wrinkled and damaged when treated with the CFS of L. plantarum LR-14. In addition, whole genome sequencing analysis indicates that L. plantarum LR-14 contains various functional genes, including predicted extracellular polysaccharides (EPS) biosynthesis genes, and genes participating in the synthesis and metabolism of fatty acid, implying that L. plantarum LR-14 has the potential to be used as a probiotic with multiple functions.
Keyphrases
- staphylococcus aureus
- lactic acid
- genome wide
- biofilm formation
- human health
- pseudomonas aeruginosa
- fatty acid
- genome wide identification
- bioinformatics analysis
- escherichia coli
- induced apoptosis
- methicillin resistant staphylococcus aureus
- candida albicans
- health risk
- gene expression
- genome wide analysis
- drinking water
- endoplasmic reticulum stress
- cell proliferation
- transcription factor