Ultra Performance Liquid Chromatography-Q Exactive Orbitrap/Mass Spectrometry-Based Lipidomics Reveals the Influence of Nitrogen Sources on Lipid Biosynthesis of Mortierella alpina.
Hengqian LuHaiqin ChenXin TangQin YangHao ZhangYong Q ChenJianxin ZhaoPublished in: Journal of agricultural and food chemistry (2019)
The objective of the present study was to reveal the effects of four types of nitrogen sources (soymeal, yeast extract, KNO3, and ammonium tartrate) on the lipid metabolism of the oleaginous fungus Mortierella alpina using untargeted lipidomics, targeted fatty acid, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. Our results showed clear differences in the contents and compositions of lipids between four types of nitrogen sources. Soymeal and ammonium tartrate supplementation favored the accumulation of triglycerides with arachidonic acid (ARA) and C16-18 fatty acids, respectively. These results were further validated by our targeted fatty acid analysis. RT-qPCR analysis of related genes in M. alpina between the four nitrogen source conditions found that soymeal supplementation dramatically increased the expression of GPAT, ELOVL, and Δ12/Δ6 desaturase. Our findings provided new insights into the regulation of lipid biosynthesis in M. alpina and potential avenues for genetic manipulation and highlighted the importance of an optimal nitrogen source for ARA-rich oil production.
Keyphrases
- fatty acid
- mass spectrometry
- liquid chromatography
- high resolution mass spectrometry
- high resolution
- tandem mass spectrometry
- drinking water
- ultra high performance liquid chromatography
- poor prognosis
- cancer therapy
- genome wide
- cell wall
- ionic liquid
- gas chromatography
- single cell
- capillary electrophoresis
- oxidative stress
- risk assessment
- simultaneous determination
- transcription factor
- drug delivery
- solid phase extraction
- gas chromatography mass spectrometry