PICKLES v3: the updated database of pooled in vitro CRISPR knockout library essentiality screens.
Lance C NovakJuihsuan ChouMedina ColicChristopher A BristowTraver HartPublished in: Nucleic acids research (2022)
PICKLES (https://pickles.hart-lab.org) is an updated web interface to a freely available database of genome-scale CRISPR knockout fitness screens in human cell lines. Using a completely rewritten interface, researchers can explore gene knockout fitness phenotypes across cell lines and tissue types and compare fitness profiles with fitness, expression, or mutation profiles of other genes. The database has been updated to include data from three CRISPR libraries (Avana, Score, and TKOv3), and includes information from 1162 whole-genome screens probing the knockout fitness phenotype of 18 959 genes. Source code for the interface and the integrated database are available for download.
Keyphrases
- genome wide
- body composition
- dna methylation
- physical activity
- copy number
- adverse drug
- crispr cas
- endothelial cells
- genome editing
- poor prognosis
- high throughput
- gene expression
- genome wide identification
- molecular dynamics simulations
- binding protein
- single molecule
- deep learning
- transcription factor
- artificial intelligence