Electroporation is a technique to introduce DNA constructs into cells using electric current. Here, we present a protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage. We describe steps for setting up and conducting electroporation. We then detail procedures for collecting, fixing, and mounting embryos and counting expression. This protocol can be used to study the expression of enhancers via reporter assays, manipulating cells using genes or modified genes such as dominant negatives, and genome editing. For complete details on the use and execution of this protocol, please refer to Song, et al. 1 .
Keyphrases
- crispr cas
- genome editing
- randomized controlled trial
- induced apoptosis
- circulating tumor
- poor prognosis
- cell free
- single molecule
- cell cycle arrest
- escherichia coli
- single cell
- genome wide
- cell therapy
- oxidative stress
- klebsiella pneumoniae
- signaling pathway
- high throughput
- gene expression
- dna methylation
- long non coding rna
- cell proliferation
- bioinformatics analysis
- circulating tumor cells