Modulation of long noncoding RNAs by risk SNPs underlying genetic predispositions to prostate cancer.
Haiyang GuoMusaddeque AhmedFan ZhangCindy Q YaoSiDe LiYi LiangJunjie HuaFraser SoaresYifei SunJens LangsteinYuchen LiChristine PoonSwneke D BaileyKinjal DesaiTeng FeiQiyuan LiDorota H SendorekMichael FraserJohn R PrensnerTrevor J PughMark PomerantzRobert G BristowMathieu LupienFelix Y FengPaul C BoutrosMatthew L FreedmanMartin J WalshHousheng Hansen HePublished in: Nature genetics (2016)
Long noncoding RNAs (lncRNAs) represent an attractive class of candidates to mediate cancer risk. Through integrative analysis of the lncRNA transcriptome with genomic data and SNP data from prostate cancer genome-wide association studies (GWAS), we identified 45 candidate lncRNAs associated with risk to prostate cancer. We further evaluated the mechanism underlying the top hit, PCAT1, and found that a risk-associated variant at rs7463708 increases binding of ONECUT2, a novel androgen receptor (AR)-interacting transcription factor, at a distal enhancer that loops to the PCAT1 promoter, resulting in upregulation of PCAT1 upon prolonged androgen treatment. In addition, PCAT1 interacts with AR and LSD1 and is required for their recruitment to the enhancers of GNMT and DHCR24, two androgen late-response genes implicated in prostate cancer development and progression. PCAT1 promotes prostate cancer cell proliferation and tumor growth in vitro and in vivo. These findings suggest that modulating lncRNA expression is an important mechanism for risk-associated SNPs in promoting prostate transformation.
Keyphrases
- prostate cancer
- radical prostatectomy
- genome wide
- transcription factor
- cell proliferation
- genome wide association
- poor prognosis
- dna methylation
- gene expression
- binding protein
- electronic health record
- signaling pathway
- long non coding rna
- machine learning
- long noncoding rna
- protein kinase
- network analysis
- smoking cessation
- replacement therapy
- genetic diversity