The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues.
Yuma WadaTetsuya IkemotoYuji MorineSatoru ImuraYu SaitoShinichiro YamadaMitsuo ShimadaPublished in: Scientific reports (2019)
The aim of this study was to investigate the characteristics of insulin producing cells (IPCs) differentiated from adipose-tissue derived stem cells (ADSCs) isolated from human subcutaneous and visceral adipose tissues and identify ADSCs suitable for differentiation into efficient and functional IPCs. Subcutaneous and visceral adipose tissues collected from four (4) patients who underwent digestive surgeries at The Tokushima University (000035546) were included in this study. The insulin secretion of the generated IPCs was investigated using surface markers by: fluorescence activated cell sorting (FACS) analysis; cytokine release; proliferation ability of ADSCs; in vitro (glucose-stimulated insulin secretion: (GSIS) test/in vivo (transplantation into streptozotocin-induced diabetic nude mice). The less fat-related inflammatory cytokines secretions were observed (P < 0.05), and the proliferation ability was higher in the subcutaneous ADSCs (P < 0.05). Insulin expression and GISI were higher in the subcutaneous IPCs (P < 0.01 and P < 0.05, respectively). The hyperglycaemic state of all mice that received IPCs from subcutaneous fat tissue converted into normo-glycaemia in thirty (30) days post-transplantation (4/4,100%). Transplanted IPCs were stained using anti-insulin and anti-human leukocyte antigen antibodies. The IPCs generated from the ADSCs freshly isolated from the human fat tissue had sufficient insulin secreting ability in vitro and in vivo.
Keyphrases
- adipose tissue
- type diabetes
- insulin resistance
- endothelial cells
- stem cells
- high fat diet
- glycemic control
- gene expression
- induced apoptosis
- induced pluripotent stem cells
- signaling pathway
- pluripotent stem cells
- cell therapy
- high fat diet induced
- newly diagnosed
- high glucose
- cell cycle arrest
- poor prognosis
- metabolic syndrome
- single cell
- skeletal muscle
- fatty acid
- ejection fraction
- bone marrow
- cell proliferation
- blood glucose
- binding protein
- endoplasmic reticulum stress
- mesenchymal stem cells
- long non coding rna
- diabetic nephropathy
- cell death
- quantum dots