UHPLC-HRMS Spectrometric Analysis: Method Validation and Plasma and Urinary Metabolite Identification after Mango Pulp Intake.
Salud Cáceres-JiménezRaquel Rodríguez-SolanaSara DobaniKirsty PourshahidiChris GillJosé Manuel Moreno-RojasTahani M AlmutairiAlan CrozierMaria Gema Pereira-CaroPublished in: Journal of agricultural and food chemistry (2023)
After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography-high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.
Keyphrases
- high resolution mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- ms ms
- tandem mass spectrometry
- ultra high performance liquid chromatography
- simultaneous determination
- gas chromatography
- mass spectrometry
- phase ii
- solid phase extraction
- clinical trial
- endothelial cells
- randomized controlled trial
- liver failure
- open label
- microbial community
- physical activity
- body mass index
- bioinformatics analysis
- aortic dissection
- double blind