Lacticaseibacillus rhamnosus Strains for Alleviation of Irritable Bowel Disease and Chronic Fatigue Syndrome.
Liang ZhangXue NiMinzhi JiangMengxuan DuShuwen ZhangHe JiangChang LiuShuang-Jiang LiuPublished in: Microorganisms (2024)
Lacticaseibacillus rhamnosus is applied as a probiotic to alleviate various metabolic, gastrointestinal, and psychological symptoms and diseases, and its probiotic effectiveness is strain-specific. In this study, we obtained 21 strains of Ls. rhamnosus , and their genomes were sequenced. We defined the pan- and core-genomes of Ls. rhamnosus . Phenotypes such as the assimilation of carbohydrates and antibiotic resistance were experimentally characterized and associated with genome annotations. Nine strains were selected and tested for growth rates, tolerance to acidity/alkalinity and bile acids, the production of short-chain fatty acids, and competition with pathogenic microbes. Strains WL11 and WL17 were targeted as potential probiotics and were applied in mouse model tests for the alleviation of chronic fatigue syndrome (CFS) and irritable bowel syndrome (IBS). The results showed that WL11 and WL17 effectively alleviated slow body weight gain, anxiety, poor memory, and cognitive impairment in CFS mouse models. They also reduced the expression of pro-inflammatory factors, such as TNF-α and IL-6, and alleviated intestinal peristalsis, visceral hypersensitivity, and anxiety-like behavior in IBS mouse models. This study reports new Ls. rhamnosus strain resources and their effect on alleviation of both IBS and CFS symptoms with mouse models; the probiotic functions of those strains in human patients remain to be further tested.
Keyphrases
- mouse model
- irritable bowel syndrome
- sleep quality
- escherichia coli
- weight gain
- cognitive impairment
- randomized controlled trial
- end stage renal disease
- endothelial cells
- fatty acid
- ejection fraction
- systematic review
- depressive symptoms
- case report
- poor prognosis
- bacillus subtilis
- prognostic factors
- cancer therapy
- insulin resistance
- binding protein
- patient reported