Mapping the metabolic characteristics of probiotic-fermented Ganoderma lucidum and its protective mechanism against Cd-induced nephrotoxicity.
Zhen DaiGaoheng LiXixi WangBo GaoXiuwei GaoPadraig StrappeZhongkai ZhouPublished in: Food & function (2023)
An animal model of Cd-induced kidney damage was designed to investigate the nephroprotective potential of the probiotic-fermented Ganoderma lucidum (FGL) via metabonomic analysis. The results showed that FGL enhanced sugar and amino acid metabolism. The interaction of Ganoderma lucidum (GL) and probiotics efficiently elevated short-chain fatty acid production following gut microbiota fermentation. The current data revealed that the FGL intervention alleviated Cd-induced nephrotoxicity via elevating the activity of antioxidant enzymes and decreasing the levels of pro-inflammatory and apoptotic factors. Based on transcriptome analysis, FGL intervention mediated renal dysfunction via decreasing the expressions of Nos2 , Tnfsf14 , S100a9 , Map3k6 and Hk3 , which were involved in oxidative stress, inflammatory response and the apoptosis process. The current study highlights a new approach for achieving positive nephroprotection via natural product intervention.
Keyphrases
- oxidative stress
- diabetic rats
- high glucose
- randomized controlled trial
- drug induced
- inflammatory response
- lactic acid
- cell death
- fatty acid
- amino acid
- ischemia reperfusion injury
- dna damage
- endothelial cells
- high resolution
- induced apoptosis
- nk cells
- mass spectrometry
- electronic health record
- signaling pathway
- big data
- lps induced
- heat stress
- artificial intelligence