In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract.
Deepti DabasRyan J EliasGregory R ZieglerJoshua D LambertPublished in: International journal of food science (2019)
Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D1 and E2 in LNCaP cells. This was associated with cell G0/G1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.
Keyphrases
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- nuclear factor
- prostate cancer
- signaling pathway
- endothelial cells
- cell cycle arrest
- squamous cell carcinoma
- poor prognosis
- bone marrow
- mesenchymal stem cells
- stem cells
- cell therapy
- mass spectrometry
- blood pressure
- smoking cessation
- cell death
- climate change
- single cell
- benign prostatic hyperplasia
- single molecule
- replacement therapy