PTEN Lipid Phosphatase Activity Enhances Dengue Virus Production through Akt/FoxO1/Maf1 Signaling.
Bin LiuTing-Ting GaoXiao-Yu FuZhen-Hao XuHao RenPing ZhaoZhong-Tian QiZhao-Ling QinPublished in: Virologica Sinica (2020)
Dengue virus (DENV) is an arthropod-borne viral pathogen and a global health burden. Knowledge of the DENV-host interactions that mediate virus pathogenicity remains limited. Host lipid metabolism is hijacked by DENV for virus replication in which lipid droplets (LDs) play a key role during the virus lifecycle. In this study, we reveal a novel role for phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in LDs-mediated DENV infection. We demonstrate that PTEN expression is downregulated upon DENV infection through post-transcriptional regulation and, in turn, PTEN overexpression enhances DENV replication. PTEN lipid phosphatase activity was found to decrease cellular LDs area and number through Akt/FoxO1/Maf1 signaling, which, together with autophagy, enhanced DENV replication and virus production. We therefore provide mechanistic insight into the interaction between lipid metabolism and the DENV replication cycle.
Keyphrases
- dengue virus
- cell proliferation
- zika virus
- pi k akt
- signaling pathway
- aedes aegypti
- fatty acid
- global health
- transcription factor
- healthcare
- cell death
- poor prognosis
- sars cov
- oxidative stress
- staphylococcus aureus
- endoplasmic reticulum stress
- single cell
- risk factors
- candida albicans
- fluorescent probe
- sensitive detection