Apoptosis Induction in Murine Melanoma (B16F10) Cells by Mannosylerythritol Lipids-B; a Glycolipid Biosurfactant with Antitumoral Activities.
Paulo Emílio FeuserAna Letícia Silva CoelhoM E de MeloRahisa ScusselBruno Augusto Mattar CarciofiRicardo Andrez Machado de ÁvilaCristiano José AndradeDébora de OliveiraPublished in: Applied biochemistry and biotechnology (2021)
Mannosylerythritol lipids have drawn attention to cosmetic and pharmaceutical industries due to their non-toxicity and excellent biological interactions with human skin, particularly with the deepest epidermal layer. Lamellar liquid crystal structure, formed by MEL-B, is an interesting feature due to its similarity to the stratum corneum molecular arrangement and cell signaling events involved in the deregulation of the cancerous cell membrane. Thus, this work aimed to evaluate the cytotoxicity of commercial mannosylerythritol lipids-B in murine melanoma, fibroblast, and human erythrocytes cells. Cytotoxic effect was more pronounced on the tumor cells from 20 µg/mL, reducing cell viability by 65%, whereas fibroblast and human erythrocytes cells were more resistant to glycolipid treatment. Fluorescence microscopy and flow cytometer proved that mannosylerythritol lipids-B is an apoptosis inducer in tumor cells related to reactive oxygen species generation.
Keyphrases
- cell cycle arrest
- induced apoptosis
- cell death
- endoplasmic reticulum stress
- oxidative stress
- endothelial cells
- pi k akt
- crystal structure
- reactive oxygen species
- single molecule
- fatty acid
- signaling pathway
- machine learning
- working memory
- deep learning
- high throughput
- induced pluripotent stem cells
- high resolution
- cell therapy
- neural network
- high speed
- smoking cessation