High-Fat Diet Augments the Effect of Alcohol on Skeletal Muscle Mitochondrial Dysfunction in Mice.
Ahmed IsmaeelJoseph A LaudatoEmma FletcherEvlampia PapoutsiAbigail TiceLara S HwaDimitrios MiserlisAthanasios Z JamurtasJennifer L SteinerPanagiotis KoutakisPublished in: Nutrients (2022)
Previous studies have shown that chronic heavy alcohol consumption and consumption of a high-fat (HF) diet can independently contribute to skeletal muscle oxidative stress and mitochondrial dysfunction, yet the concurrent effect of these risk factors remains unclear. We aimed to assess the effect of alcohol and different dietary compositions on mitochondrial activity and oxidative stress markers. Male and female mice were randomized to an alcohol (EtOH)-free HF diet, a HF + EtOH diet, or a low-Fat (LF) + EtOH diet for 6 weeks. At the end of the study, electron transport chain complex activity and expression as well as antioxidant activity and expression, were measured in skeletal muscles. Complex I and III activity were diminished in muscles of mice fed a HF + EtOH diet relative to the EtOH-free HF diet. Lipid peroxidation was elevated, and antioxidant activity was diminished, in muscles of mice fed a HF + EtOH diet as well. Consumption of a HF diet may exacerbate the negative effects of alcohol on skeletal muscle mitochondrial health and oxidative stress.
Keyphrases
- oxidative stress
- skeletal muscle
- weight loss
- physical activity
- alcohol consumption
- insulin resistance
- risk factors
- high fat diet induced
- adipose tissue
- poor prognosis
- acute heart failure
- public health
- dna damage
- randomized controlled trial
- mental health
- type diabetes
- heart failure
- fatty acid
- radiation therapy
- wild type
- signaling pathway
- climate change
- heat shock
- gestational age