Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
Marion ArnaudJohanna ChiffelleRaphael GenoletBlanca Navarro RodrigoMarta A S PerezFlorian HuberMorgane MagninTu Nguyen-NgocPhilippe GuillaumePetra BaumgaertnerChloe ChongBrian J StevensonDavid GfellerMelita IrvingDaniel E SpeiserJulien SchmidtVincent ZoeteLana Elias KandalaftMichal Bassani-SternbergSara BobisseGeorge CoukosAlexandre HarariPublished in: Nature biotechnology (2021)
The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice.