Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.

Adam M ThomasSanja BrolihJoanna F McGouran Afaf Helmy El-SagheerDenis PtchelkineMorgan JonesNeil Q McDonaldPeter J McHugh Tom Brown

Published in: Chemical communications (Cambridge, England) (2019)

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

Keyphrases

dna repair
drug discovery
high throughput
dna damage
dna damage response
small molecule
single molecule
single cell
case control
oxidative stress